Cell Culture Workflow

There are two main ways of obtaining and culturing cells: isolating them from a cell bank or donor tissue. Cells obtained from a cell bank are thawed and then seeded, and the cell culture workflow, including seeding, cell expansion, harvesting, detection, analysis, and storage, is described as an example of the process of obtaining cells from a cell bank. Each step of the process can only be achieved with the assistance of instruments.

Shop by Cell Culture Workflow Steps

Step 1: Thawing

Cell banks often store cells in liquid nitrogen containers. We need to quickly transfer frozen cells stored in liquid nitrogen containers or ultra low temp freezer to a 37°C water bath until the cells are thawed. Liquid nitrogen tanks are usually used for transporting or storing liquid nitrogen for frozen cells.

Cryobiobank Liquid Nitrogen Tank

Portable Storage Liquid Nitrogen Tank

Static Storage Liquid Nitrogen Tank

Step 2: Seeding

Cell seeding is the first step in cell culture and refers to the process of inoculating cells onto a petri dish or culture dish. This process is a crucial part of cell culture and has a significant impact on the reproducibility and accuracy of cell experiments. Cell seeding will use pipettes, centrifuge tubes and so on.

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Pipette

Manual Volume Pipettes, 12 Channel

Volume Range : 0.5μL ~ 300μL
Increment : 0.1 ~ 5μL
Test Volume : 1μL ~ 300μL

Single-channel Electronic Pipette

Volume Range : 0.5μL ~ 1000μL
Increment : 0.01μL ~ 5μL
Test Volume : 1μL ~ 1000μL

Eight-channel Electronic Pipette

Volume Range : 0.5μL ~ 300μL
Increment : 0.01μL ~ 1μL
Test Volume : 1μL ~ 300μL

Step 3: Cell Expansion

Cell Expansion is used to grow more cells, which is a key part of cell growth. Cells are expanded to a sufficient number before they can be used for various experiments and research. Proper cell expansion ensures a high quality cell product and provides a solid foundation for successful cell-related applications. Before starting, various expansion products need to be prepared, including media, culture flasks, and shaker.

Culture media

Culture flask

Culture flasks

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Single-layer Shaker

Display Mode : LCD 
Circulation Mode : Natural Convection
Speed Range (r/min) : 30-300
Timing Range(min) : 0-999.59
Size of Rocking Plate(mm) : 680×500 / 840×620 / 1108×740

Stackable CO2 Incubator

Capacity : 170L / 208L / 282L
Temperature Range : 4°C-60°C
Sterilization Mode : Ultraviolet sterilization
CO2 Control Scope : 0-20%
Humidification Mode : Natural evaporation

IR Sensor CO2 Incubator

Chamber Volume(L) : 170
Temperature Range(℃) : RT+3～55
CO2  Range : 0～20%
Sterilization : 180°C dry heat
Humidity Method : Integrated pan

Step 4: Harvesting

Collecting and isolating cells may involve centrifugation, depth filtration, and cell separation. Adherent cells also need to be separated from the surface of the cell culture flask or dish before proceeding. Cell scrapers and centrifuges may be used in the Harvesting process for cell culture.

4000RPM Low Speed Centrifuge

5000RPM Low Speed Centrifuge

6000RPM Low Speed Centrifuge

Step 5: Testing

Once the cells have been harvested, they need to be tested for characteristics such as viability, purity, and quantity. To ensure a more efficient process and more accurate results, you will need to purchase a counter, reader, etc. to test the cells.

Automatic Colony Counter by Image Analysis

Automatic Bacterial Colony Counter

Automatic Elisa Plate Reader

Step 6: Analyse

Start studying your cells by observing and analyzing them. With the right equipment, you can make this step of your workflow more efficient. Choose from a wide range of microscopes, microplate washers and readers for cell culture analysis.

Inverted Microscope

Biological Microscope

Precision Washing Elisa Plate Washer

Step 7: Storage

There are many product options and technologies available when storing cells. For example, cryopreservation can help keep your cells safe for the long term for future research projects. Find vials, microplates, and media for cell storage.