Protein Biology Methods

Genes guide the production of proteins, which help us to diagnose diseases better and develop drugs through protein isolation, purification and detection. Protein biology helps us understand these genes' output and how they affect our health. In this article, we will explore some of the key protein biology approaches that have revolutionized the field and continue to drive groundbreaking discoveries.

1. Isolation

2. Pupification

3. Separation

4. Detection

Steps to Protein Biology Methods

Step 1: Isolation

Use the following products to isolate proteins from a mixture. This protein biology approach will help you start your process and prepare for future workflow steps. Isolation of proteins typically involves multiple steps, including cell fragmentation, protein extraction, and purification. Below, you will find the necessary equipment for protein extraction and other products and resources.

Step 2: Purification

Purification is another essential protein biology method involving cell lysis, protein binding, washing and elution. The protocol used depends on the protein you want to purify. Firstly, purchase pre-loaded columns, products for protein purification, dialysis and desalting, etc.

Step 3: Separation

Solubility, size, charge and other protein characteristics are used to separate proteins. In addition to electrophoresis and dialysis, these techniques include various forms of chromatography. This process typically uses products such as browse gels, columns and reagents.

Step 4：Detection

Protein blotting and ELISA techniques are frequently used for protein detection. Fluorescence imaging microscopy is also common.

Purchasing Lab Equipment by Protein Biology Methods

Centrifuge

Protein Micro Centrifuge

Max speed: 18500r/min
Max RCF: 23797×g
Max capacity: 100ml×4
Speed accuracy: ± 30r/min

Histological Micro Centrifuge

Max speed: 15000r/min
Max RCF: 21380×g
Max capacity: 5ml×18
Speed accuracy: ± 20r/min

Multipurpose Centrifuge

Max speed: 20500r/min
Max RCF: 29200×g
Max capacity: 750ml×4
Speed accuracy: ± 50r/min

Electrophoresis

Vertical Electrophoresis Tank

Sample Comb : 10 samples
Number of Gels : 1-4 pcs
Number of  Samples : 10~40pcs
Buffer Capacity : 1000ml

Horizontal Electrophoresis Tank

Sample Comb : 26, 52 samples
Number of Gels : 1-6 pcs
Number of Samples : 360pcs
Buffer Capacity : 1600ml

Vertical Electrophoresis Tank

Sample Comb : 68, 102 samples
Gel thickness : 1.0mm
Number of Samples : 200pcs
Buffer Capacity : 800ml

Chromatograph

Liquid Chromatograph

HPLC Chromatograph

Automatic HPLC Chromatograph