Views: 1021 Author: Yammi Publish Time: 2024-10-21 Origin: Site
Polymerase Chain Reaction (PCR) and Quantitative PCR (qPCR) are methods used to replicate genetic material in order to detect small amounts of it. Whether you are performing mutagenesis experiments, cloning DNA, performing forensic or parental testing, or detecting pathogens, you can find the premixes, polymerases, dyes, primers, and thermal cyclers you need to complete your workflow.
Shop by PCR and qPCR Methods
Sample Preparation
Preparation of samples for PCR or qPCR usually requires extraction and purification of nuclear material. Depending on the source of the sample, the sample is prepared with the help of centrifuges and precision pipettes, etc., prior to qPCR or PCR.
Amplification is performed by combining DNA fragments, polymerase, nucleotide solution and primers in PCR tubes or microtiter plates. The following steps are performed using a thermal cycler and are repeated 35 to 40 times.
Denaturation: Hydrogen bond breaking, DNA separation
Annealing: Primers and polymerase bind to DNA
Extension: Nucleotides pair with DNA to form new complementary strands
In real-time PCR, RNA is converted to cDNA and then amplified using PCR. Real-time PCR is used to detect specific DNA sequences. The method uses a fluorescent dye or probe to determine the amount of amplified material in the sample. Find a variety of premixes as well as real-time PCR instruments, assays, and kits for quantifying qPCR results.
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