Chromatographic Columns - Detection of Chemical Substance in Food/Medicine
Views: 124 Author: Yammi Publish Time: 2023-12-28 Origin: Site
Chromatographic columns are an important part of a chromatograph, and they come in various types. Each column type is designed for specific analytical needs and plays an important role in different fields. The two most common examples of stationary phases for column chromatography are silica gel and alumina, while organic solvents are considered the most common mobile phase. Let's explore the principles of chromatographic columns, composition, preservation, and applications.
Principle of Chromatographic Columns
The principle of a chromatographic column is to separate a mixture into separate parts with the help of stationary adsorption of solutes from the solution. As the mixture passes through the column, the compounds interact with the packing material and are separated according to their relative affinity or repulsion to the packing material. This separation process can be based on principles such as adsorption, partitioning, ion exchange or chiral recognition, depending on the column type and the packing material's nature.
Chromatographic Columns
Scitek chromatographic columns are available in various sizes to meet customers' needs in various fields. Scitek's CV, DX, and S series are made of ultra-pure spherical silica gel as the substrate. They are carefully manufactured using unique silica gel surface bonding technology and column loading process. They are excellent in selectivity, stability, versatility and other chromatographic properties.
Features:
Ultra-pure spherical silica gel purity＞99.9%
Accommodate different separation and analysis needs
Unique stationary phase bonding technology
Excellent batch size
Wide range of stationary phases
High column efficiency
CV Series Liquid Chromatography
This series of columns is the first choice for separation in routine liquid chromatography, with excellent chromatographic performance showing good separation results in routine sample analysis.
The QA Series are polar reversed-phase liquid chromatography columns that improve the retention of polar compounds and provide different selectivity for the separation of polar compounds.
Application 1: Determination of aspalathin in Tianma headache tablets
Chromatographic separations often encounter problems that are difficult to solve with conventional columns, such as difficulty in controlling the peak shape of basic compounds, abnormal chromatographic retention of strongly polar compounds such as sugars, and poor column tolerance under pure water conditions.
DX-I series adopts a unique amide bonding technology with polar group connection, and its high hydrophilicity and stability are suitable for hydrophilic chromatography and forward chromatographic separation. With excellent retention and selectivity, they are suitable for separating oligosaccharides, peptides and organic acids.
Application 1: Detection of Barberry Oligosaccharides
DX-II series improves the retention of polar compounds while providing different selectivity for the separation of polar compounds, taking into account the retention and separation of both polar and non-polar compounds.
Application 1: Fingerprints of Scutellaria Scutellariae Lian Tang
DX-HC series of positive charge-modified reverse chromatography columns use a unique "polar copolymerization" technology to introduce a positive charge into the reversed-phase bonded silica gel, which achieves a balanced regulation of the shielding and electrostatic effects of the active silanol groups on the surface of the silica gel. The problem of peak trailing in the spectrum of alkaline compounds is fundamentally solved, thus ensuring the perfect symmetry of alkaline compounds.
S Series columns are customized for specific separations and provide a proprietary solution for a particular sample or research project.
The S-PFP Chromatography Packing has a unique surface-bonded phase treatment, resulting in strong dipole induction. It is widely used in the separation and analysis of isomers because of its excellent separation of easily polarised substances such as aromatic, heterocyclic, and halides.
Core Series columns have a core-shell silica particle structure consisting of solid silica spheres with a porous surface. This core-shell structure provides a shorter mass transfer path for separating substances and reduces axial diffusion.
This core-shell structure provides a shorter mass transfer path for material separation, reduces axial diffusion, increases column efficiency, and provides better separation for complex samples.
The solid core provides a robust support structure that can withstand high pressures and significantly reduces column backpressure.
Feature:
Multiple bonding phases available
Higher column efficiency and lower back pressure
Provides intentional peak shapes
High resolution and efficiency
C81 bonded columns have shown stable retention performance for traditional Chinese medicine and chemical drugs.
Prepared Codonopsis Extract - Alkaloids Compounds
Candesartan Extract - Carboxylic Acid Esters
Wild Chrysanthemum Extract - Carboxylates
Cefaclor Extract - Antibiotics
C8 column is an octyl-bonded phase with a unique tailing technology, which effectively reduces the silanol group activity on the surface of the packing material and provides better peak shapes for the analytes.
C81 QA column packing is embedded with a unique polarity gene, which is suitable for 100% spring water mobile phase, overcoming the "hydrophobic collapse" problem of bonded phase; good retention of strong polar compounds.
Application 1: Detection of small molecule organic acids
Check the arrow mark of the liquid flow direction on the column before installation, and install the column according to the direction; avoid reverse installation.
Screw the connectors of the tubing into the ends of the column, and the tubing connectors should be tightly connected to the column to ensure that there is zero dead volume and that there is no leakage of liquid during the operation of the chromatograph; o Use the recommended pH and temperature.
Use the column within the recommended pH and temperature ranges, as exceeding the pH and temperature ranges will shorten the column's life.
Do not drop the column or hit it with hard objects, as peak cracking may occur when it is hit.
When using organic solvent-buffer system as mobile phase, make sure that the buffer salts will not precipitate out during the whole method run.
As a professional manufacturer, Scitek Global is certified by ISO 9001, ISO 13485, ISO 45001 and ISO 14001. Almost all our products are certified by ETL, CE and FDA .
Scitek Global looks forward to establishing cooperation with more distributors all over the world, and working together to create greater customer value.
