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Chromatographic Columns - Detection of Chemical Substance in Food/Medicine

Views: 124     Author: Yammi     Publish Time: 2023-12-28      Origin: Site

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Chromatographic columns are an important part of a chromatograph, and they come in various types. Each column type is designed for specific analytical needs and plays an important role in different fields. The two most common examples of stationary phases for column chromatography are silica gel and alumina, while organic solvents are considered the most common mobile phase. Let's explore the principles of chromatographic columns, composition, preservation, and applications.


Principle of Chromatographic Columns


The principle of a chromatographic column is to separate a mixture into separate parts with the help of stationary adsorption of solutes from the solution. As the mixture passes through the column, the compounds interact with the packing material and are separated according to their relative affinity or repulsion to the packing material. This separation process can be based on principles such as adsorption, partitioning, ion exchange or chiral recognition, depending on the column type and the packing material's nature.


Chromatographic Columns


Scitek chromatographic columns are available in various sizes to meet customers' needs in various fields. Scitek's CV, DX, and S series are made of ultra-pure spherical silica gel as the substrate. They are carefully manufactured using unique silica gel surface bonding technology and column loading process. They are excellent in selectivity, stability, versatility and other chromatographic properties.

Features:

  1. Ultra-pure spherical silica gel purity>99.9%

  2. Accommodate different separation and analysis needs

  3. Unique stationary phase bonding technology

  4. Excellent batch size

  5. Wide range of stationary phases

  6. High column efficiency


CV Series Liquid Chromatography


This series of columns is the first choice for separation in routine liquid chromatography, with excellent chromatographic performance showing good separation results in routine sample analysis.


cv-kangshengsu (1)

Application 1: Detection of quinolone antibiotics

Chromatography Column: 4.6x250mm, 5µm, 100Å
Mobile Phase: Phosphoric acid/aqueous solution-acetonitrile
Detection Wavelength: 278 nm
Injection Volume: 10 µL


cv-heshouwu (1)

Application 2: Characterisation of HeShouWu Granules

Chromatography Column: 4.6x250mm, 5µm, 100Å
Mobile Phase: Phosphoric acid/aqueous solution-acetonitrile
Detection Wavelength: 254 nm
Injection Volume: 10 µL

cv-shuangmidamopian (1)

Application 3: Detection of Dipyridamole Tablets

Chromatography Column: 4.6x250mm, 5µm, 100Å
Mobile Phase: Disodium hydrogen phosphate solution - methanol
Detection Wavelength: 288 nm
Injection Volume: 20 µL


QA Series Liquid Chromatography


The QA Series are polar reversed-phase liquid chromatography columns that improve the retention of polar compounds and provide different selectivity for the separation of polar compounds.


cv-tianmatoutongpian (1)

Application 1: Determination of aspalathin in Tianma headache tablets

Chromatography Column: 4.6x250mm, 5µm, 100Å
Mobile Phase: Acetonitrile-water
Detection Wavelength: 220 nm
Injection Volume: 10 µL

cv-xiyangshen (1)

Application 2: Determination of ginseng content

Chromatography Column: 4.6x250mm, 5µm, 100Å
Mobile Phase: Acetonitrile-0.1% phosphoric acid solution
Detection Wavelength: 203 nm
Injection Volume: 10 µL

cv-fructose (1)

Application 2: Detection of fructose, glucose, sucrose, maltose

Chromatography Column: 4.6x250mm, 5µm, 100Å
Mobile Phase: Acetonitrile-water
Differential Detector Temperature: 40℃
Injection Volume: 20 µL


DX Series Liquid Chromatography


Chromatographic separations often encounter problems that are difficult to solve with conventional columns, such as difficulty in controlling the peak shape of basic compounds, abnormal chromatographic retention of strongly polar compounds such as sugars, and poor column tolerance under pure water conditions.

DX-I series adopts a unique amide bonding technology with polar group connection, and its high hydrophilicity and stability are suitable for hydrophilic chromatography and forward chromatographic separation. With excellent retention and selectivity, they are suitable for separating oligosaccharides, peptides and organic acids.


dx-bajitianguatang (1)

Application 1: Detection of Barberry Oligosaccharides

Chromatography Column: 4.6x150mm, 5µm, 100Å
Mobile Phase: Acetonitrile-water
Flow Rate: 1.0 mL/min
Injection Volume: 20 µL


DX-II series improves the retention of polar compounds while providing different selectivity for the separation of polar compounds, taking into account the retention and separation of both polar and non-polar compounds.


dx-gegen (1)

Application 1: Fingerprints of Scutellaria Scutellariae Lian Tang

Chromatography Column: 4.6x150mm, 5µm, 100Å
Mobile Phase: Acetonitrile-formic acid-triethylamine aqueous solution
Detection Wavelength: 250 nm
Injection Volume: 10 µL


dx-organic acid (1)

Application 2: Detection of small molecule organic acids

Chromatography Column: 4.6x150mm, 5µm, 100Å
Mobile Phase: Trifluoroacetic acid/water-trifluoroacetic acid/acetonitrile
Detection Wavelength: 215 nm
Injection Volume: 10 µL



DX-HC series of positive charge-modified reverse chromatography columns use a unique "polar copolymerization" technology to introduce a positive charge into the reversed-phase bonded silica gel, which achieves a balanced regulation of the shielding and electrostatic effects of the active silanol groups on the surface of the silica gel. The problem of peak trailing in the spectrum of alkaline compounds is fundamentally solved, thus ensuring the perfect symmetry of alkaline compounds.


dx-alkali compound (1)

Application 1: Analysis of alkaline compounds

Chromatography Column: 4.6x150mm, 5µm, 100Å
Mobile Phase: Formic acid solution-acetonitrile
Detection Wavelength: 260 nm
Injection Volume: 10 µL

dx-amoxicillin (1)

Application 2: Amoxicillin, cefadroxil, cefaclor, ampicillin assays

Chromatography Column: 4.6x250mm, 5µm, 100Å
Mobile Phase: Formic acid/acetonitrile-formic acid aqueous solution
Detection Wavelength: 254 nm
Injection Volume: 10 µL



S Series Liquid Chromatography


S Series columns are customized for specific separations and provide a proprietary solution for a particular sample or research project.

The S-PFP Chromatography Packing has a unique surface-bonded phase treatment, resulting in strong dipole induction. It is widely used in the separation and analysis of isomers because of its excellent separation of easily polarised substances such as aromatic, heterocyclic, and halides.


saaa-4ve (1)

Application 1: Detection of 4 type Vitamin E 

Chromatography Column: 4.6x150mm, 5µm, 100Å
Mobile Phase: Formic acid-water
Detection Wavelength: 294 nm
Sample: Milk

saaa-19 (1)

Application 2: Detection of 19 amino acids

Chromatography Column: 4.6x250mm, 5µm, 100Å
Mobile Phase: Phosphate aqueous solution - acetonitrile
Detection Wavelength: 360 nm
Injection Volume: 10 µL



(1:Aspartic acid 2:Glutamic acid 3:Serine 4:Arginine 5:Glycine 6:Threonine 7:Proline 8:Alanine 9:Valine 10:Methionine 11:Cystine 12:Isoleucine 13:Leucine 14:Tryptophan 15:Histidine 16:Phenylalanine 17:Cysteine 18:Lysine 19:Tyrosine a,b,c were introduced as derivatives)


Core Series Liquid Chromatography


Core Series columns have a core-shell silica particle structure consisting of solid silica spheres with a porous surface. This core-shell structure provides a shorter mass transfer path for separating substances and reduces axial diffusion.

This core-shell structure provides a shorter mass transfer path for material separation, reduces axial diffusion, increases column efficiency, and provides better separation for complex samples.

The solid core provides a robust support structure that can withstand high pressures and significantly reduces column backpressure.

Feature:

  1. Multiple bonding phases available

  2. Higher column efficiency and lower back pressure

  3. Provides intentional peak shapes

  4. High resolution and efficiency

C81 bonded columns have shown stable retention performance for traditional Chinese medicine and chemical drugs.


c18-zhicaowu (1)

Prepared Codonopsis Extract - Alkaloids Compounds

c18-kandisha (1)

Candesartan Extract - Carboxylic Acid Esters

C18-YEJU (1)

Wild Chrysanthemum Extract - Carboxylates

c18-toubaotiquwu (1)

Cefaclor Extract - Antibiotics

C8 column is an octyl-bonded phase with a unique tailing technology, which effectively reduces the silanol group activity on the surface of the packing material and provides better peak shapes for the analytes.


c8-aomeilatuo (1)

Application 1: Detection of Omeprazole

Chromatography Column: 2.1x100mm, 2.6µm
Mobile Phase: Disodium hydrogen phosphate solution - acetonitrile
Detection Wavelength: 280 nm
Injection Volume: 2 µL


C81 QA column packing is embedded with a unique polarity gene, which is suitable for 100% spring water mobile phase, overcoming the "hydrophobic collapse" problem of bonded phase; good retention of strong polar compounds.


c8-xiaofenzi (1)Application 1: Detection of small molecule organic acids

Chromatography Column: 2.1x100mm, 2.6µm
Mobile Phase: Trifluoroacetic acid aqueous solution
Detection Wavelength: 215 nm
Injection Volume: 2 µL


Amide is an amide-bonded phase column that is hydrophilic and has good retention of polar compounds.


amide-guoguatang (1)

Application 1: Detection of fructooligosaccharides

Chromatography Column: 2.1x100mm, 2.6µm
Mobile Phase: 
Acetonitrile-water
Flow Rate: 0.4mL/min
Injection Volume: 2 µL




Chromatographic Column Usage Precautions



  1. Check the arrow mark of the liquid flow direction on the column before installation, and install the column according to the direction; avoid reverse installation.

  2. Screw the connectors of the tubing into the ends of the column, and the tubing connectors should be tightly connected to the column to ensure that there is zero dead volume and that there is no leakage of liquid during the operation of the chromatograph; o Use the recommended pH and temperature.

  3. Use the column within the recommended pH and temperature ranges, as exceeding the pH and temperature ranges will shorten the column's life.

  4. Do not drop the column or hit it with hard objects, as peak cracking may occur when it is hit.

  5. When using organic solvent-buffer system as mobile phase, make sure that the buffer salts will not precipitate out during the whole method run.



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